Soil and Sediment Samples Extraction and Purification Procedures for Evaluation and Optimization of DNA
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منابع مشابه
Comparative Studies on the Extraction of Metagenomic DNA from Various Soil and Sediment Samples of Jammu and Kashmir Region in Prospect for Novel Biocatalysts
A modified and efficient method has been developed for extracting high molecular weight (HMW) DNA free of inhibitors (humic substances) from soil and sediment samples of Jammu and Kashmir region. The method includes precipitation of extracted soil and sediment DNA with 0.5 volumes of 50% polyethylene glycol (PEG) and 0.1 volumes of 1M NaCl (sodium chloride) followed by treatment with 2% CaCl2( ...
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We compared and statistically evaluated the effectiveness of nine DNA extraction procedures by using frozen and dried samples of two silt loam soils and a silt loam wetland sediment with different organic matter contents. The effects of different chemical extractants (sodium dodecyl sulfate [SDS], chloroform, phenol, Chelex 100, and guanadinium isothiocyanate), different physical disruption met...
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Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...
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Complexity and heterogeneity of soil samples have often implied the inclusion of purification steps in conventional DNA extraction for polymerase chain reaction (PCR) assays. Unfortunately the purification steps are also time and labor intensive. Therefore the necessity of DNA purification was re-visited and investigated for a variety of environmental soil samples that contained various amounts...
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تاریخ انتشار 1999